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I4144
Sigma
Interleukin-3 from mouse
lyophilized powder, recombinant, expressed in Escherichia coli, cell culture tested
| Synonym: | Interleukin-3 from mouse |
| MDL number: | MFCD00164817 |
Description
| Analysis Note | The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells. |
| Biochem/physiol Actions | Interleukin-3 is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor. |
| Physical form | Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | ≥98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| potency | 0.01-0.1 ng/mL ED50/EC50 |
| mol wt | mol wt 15.1 kDa |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| reference | Cerny, J., et al., Stimulation of bone marrow haemopoietic stem cells by a factor from activated T cells. Nature 249, 63-66, (1974) |
| Luger, T., et al., Epidermal cells synthesize a cytokine with interleukin 3-like properties. J. Immunol. 134, 915-919, (1985) | |
| Schrader, J., et al., P-cell stimulating factor: characterization, action on multiple lineages of bone-marrow-derived cells and role in oncogenesis. Immunol. Rev. 76, 79-104, (1983) | |
| Santoli, D., et al., Amplification of IL-2-driven T cell proliferation by recombinant human IL-3 and granulocyte-macrophage colony-stimulating factor. J. Immunol. 141, 519-526, (1988) | |
| Tadmori, W., et al., Human recombinant IL-3 stimulates B cell differentiation. J. Immunol. 142, 1950-1955, (1989) | |
| Schrader, J., et al., The panspecific hemopoietin of activated T lymphocytes (interleukin-3). Annu. Rev. Immunol. 4, 205-230, (1986) |
